Revisiting 1,3,4-Oxadiazol-2-ones: Utilization in the Development of ABHD6 Inhibitors

Bioorg Med Chem. 2015 Oct 1;23(19):6335-45. doi: 10.1016/j.bmc.2015.08.030. Epub 2015 Aug 28.

Abstract

This article describes our systematic approach to exploring the utility of the 1,3,4-oxadiazol-2-one scaffold in the development of ABHD6 inhibitors. Compound 3-(3-aminobenzyl)-5-methoxy-1,3,4-oxadiazol-2(3H)-one (JZP-169, 52) was identified as a potent inhibitor of hABHD6, with an IC₅₀ value of 216 nM. This compound at 10 μM concentration did not inhibit any other endocannabinoid hydrolases, such as FAAH, MAGL and ABHD12, or bind to the cannabinoid receptors (CB₁ and CB₂). Moreover, in competitive activity-based protein profiling (ABPP), compound 52 (JZP-169) at 10 μM selectively targeted ABHD6 of the serine hydrolases of mouse brain membrane proteome. Reversibility studies indicated that compound 52 inhibited hABHD6 in an irreversible manner. Finally, homology modelling and molecular docking studies were used to gain insights into the binding of compound 52 to the active site of hABHD6.

Keywords: 1,3,4-Oxadiazol-2-ones; 2-Arachidonoylglycerol; Fatty acid amide hydrolase; Monoacylglycerol lipase; α/β-Hydrolase domain-containing 6.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites
  • Catalytic Domain
  • Enzyme Inhibitors / chemistry*
  • Enzyme Inhibitors / metabolism
  • Mice
  • Molecular Docking Simulation
  • Monoacylglycerol Lipases / antagonists & inhibitors*
  • Monoacylglycerol Lipases / metabolism
  • Oxadiazoles / chemistry*
  • Oxadiazoles / metabolism
  • Protein Binding
  • Receptors, Cannabinoid / chemistry
  • Receptors, Cannabinoid / metabolism
  • Serine Proteases / chemistry
  • Serine Proteases / metabolism
  • Structure-Activity Relationship

Substances

  • 1,3,4-oxadiazol-2(3H)-one
  • Enzyme Inhibitors
  • Oxadiazoles
  • Receptors, Cannabinoid
  • ABHD6 protein, mouse
  • Monoacylglycerol Lipases
  • Serine Proteases